polymerase chain reaction typing of pasteurella multocida capsules isolated in iran
Authors
abstract
capsules from a range of pathogenic bacteria are the key determinants of virulency. the capsule hasbeen implicated in virulence of pasteurella multocida. in this study a type-specific polymerase chain reaction(pcr) assay was used for capsular typing of 39 avian p. multocida isolates from iran. the pcr amplified afragment of 1044 bp from all of tested isolates. it was found that all avian p. multocida isolates belonged tocapsular type a. the sequence alignment of the fragment showed a high similarity (>96%) with thepublished sequences of p. multocida hya gene in the gene bank. it was recognised that p. multocida capsulargroup a is the dominant cause of fowl cholera in iran.
similar resources
Polymerase chain reaction typing of Pasteurella multocida capsules isolated in Iran
Capsules from a range of pathogenic bacteria are the key determinants of virulency. The capsule hasbeen implicated in virulence of Pasteurella multocida. In this study a type-specific polymerase chain reaction(PCR) assay was used for capsular typing of 39 avian P. multocida isolates from Iran. The PCR amplified afragment of 1044 bp from all of tested isolates. It was found that all avian P. mul...
full textCharacterization, ribotyping and capsular PCR typing of Pasteurella multocida isolated from Iranian sheep and goats
Pasteurella multocida is considered to be an important cause of ovine pneumonia and results causes considerable economic losses in Iran. Ribotyping PCR (Ribo-PCR) was used for investigating the diversity of ovine and caprine P.multocida. A total of 120 swab tonsil and nasal samples were obtained from sheep and goats. They were analyzed by biochemical tests and Ribo-PCR 16S-23S ribosomal RNA gen...
full textMolecular identification and capsular typing of Pasteurella multocida isolates from sheep pneumonia in Iran
Pasteurella multocida is known as one of the main organisms causing pneumonia in sheep. As immunity in pasteurellosis is serogroup specific, identification of prevalent capsular group among endemic areas is essential. The aim of this study was to molecular identification and determine of the capsular type of the P. multocida strains isolated from sheep pneumonia in Iran. Bacteriological and bio...
full textMolecular identification and capsular typing of Pasteurella multocida isolates from sheep pneumonia in Iran
ARTICLE INFO ABSTRACT Article history: Received: 2 May 2014 Accepted: 12 August 2014 Available online: 1 December 2014 Pasteurella multocida is known as one of the main organisms causing pneumonia in sheep. As immunity in pasteurellosis is serogroup specific, identification of prevalent capsular group among endemic areas is essential. The aim of this study was to molecular identification and de...
full textMy Resources
Save resource for easier access later
Journal title:
iranian journal of veterinary researchPublisher: shiraz university
ISSN 1728-1997
volume 7
issue 3 2006
Keywords
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023